Synthesis And Optimization of Streptomycin Loaded Chitosan-Alginate Nanoparticles

Nanoformulation consisting of Streptomycin loaded chitosan-alginate nanoparticles were prepared using ionotropic-pregelation method and optimization was done in terms of polymer concentration, crosslinker concentration and stirring time. The optimal parameters were found to be Chitosan 0.75mg/ml, calcium chloride 1% (w/v) and stirring time 90 min. Polymer (chitosan and sodium-alginate) and crosslinker (calcium-chloride) at these concentrations had significant synergistic effect on particle size and % encapsulation efficiency. Increase in polymer and crosslinker concentration resulted in an increase in particle size. Encapsulation efficiency, first showed an increase followed by a decrease, on increasing the polymer concentration whereas it increased with an increase in cross linker concentration. The nanoformulation so formed showed particle size 328.4 nm & drug encapsulation 93.32%.